Itching during the night in a child’s perianal area strongly suggests pinworm infection. Diagnosis is made by identifying the worm or its eggs.
If your child has a pinworm infection, you can see worms on the skin near the anal region or on underwear, pyjamas or sheets, about two or three hours after your child has fallen asleep. You also might see the worms in the toilet after your child goes to the bathroom. They look like tiny pieces of white thread and are really small — about as long as a staple. You might also see them on your child's underwear in the morning.
Pinworm eggs can be collected and examined using the “tape test” as soon as the person wakes up. This “test” is done by firmly pressing the adhesive side of clear, transparent cellophane tape to the skin around the anus. The eggs stick to the tape and the tape can be placed on a slide and looked at under a microscope. This test should be done as soon as the person wakes up in the morning before they wash, bathe, go to the toilet, or get dressed. The “tape test” should be done on three consecutive mornings to increase the chance of finding pinworm eggs.