TISSUE PREPARATION FOR POLYMERASE CHAIN REACTION (PCR)/MOLECULAR

Lab Section Category

Histopathology

View write-up

Description

Tests perform by Histomolecular and molecular lab:

EGFR Sanger Sequencing, EGFR COBAS Real time PCR, KRAS/BRAF, NRAS, BRAF V600E, BRAF Sanger Sequencing, MSI PCR, C-KIT (Exon 9, 11), C-KIT (Exon 13, 17), PDGFRA, T & B Cell Clonality test, TB PCR, SSTP(Somatic Solid Tumour Panel)

Refer to Immunohistochemistry for creating CPOE order.
Requirements for external materials for Molecular test:
For requests from external institutes, please raise a memo with the same information as above in point 1 and a copy of pathology report. Please indicate the billing location.
Upon receiving or retrieving of sample, Histopathology lab takes 4-5 working days to submit the sample to the respective laboratory for testing. Please check with the respective laboratory (Molecular lab, Translational Pathology Centre, Cytogenetic lab and Central TB lab) for turnaround time of individual test performed by them.

Sample requirements:

Test	Testing Laboratory	Sample requirements	Tumour content
EGFR Sanger Sequencing	SGH Molecular Lab	1 HE, 8 unstains at 8µm for resections, non coated slides	Minimum 30% tumour content
EGFR Sanger Sequencing	SGH Molecular Lab	1 HE, 12 unstains at 5µm for biopsies, non coated slides	Minimum 30% tumour content
EGFR Real Time PCR (cobas)	SGH Molecular Lab	1 HE, 8 unstains at 8µm for resections, non coated slides	Minimum 10% tumour content
EGFR Real Time PCR (cobas)	SGH Molecular Lab	1 HE, 12 unstains at 5µm for biopsies, non coated slides	Minimum 10% tumour content
KRAS/BRAF	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
NRAS	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
BRAF V600E	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
BRAF Sanger Sequencing	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
MSI PCR	SGH Molecular Lab	Please provide both tumour and normal tissue	Minimum 30% tumour content
		1 HE, 8 unstains at 8µm for normal
		1 HE, 8 unstains at 8µm for tumour
C-KIT (Exon 9,11)	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
C-KIT (Exon 13, 17)	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
PDGFRA	SGH Molecular Lab	Refer to requirement for EGFR	Minimum 30% tumour content
T or B Cell Clonality	SGH Molecular Lab	1 HE, 8 unstains at 8µm for resections	Minimum 1% lymphocyte content
T or B Cell Clonality	SGH Molecular Lab	1 HE, 20 unstains at 5µm for biopsies/ bone marrows	Minimum 1% lymphocyte content
1p19q FISH	Cytogenetic Lab	1HE, 4 unstains on coated slides	Minimum 100 nuclei
Lung Cancer FISH Panel	Cytogenetic Lab	1HE, 8 unstains at 4µm on coated slides	Minimum 100 nuclei
Her-2 FISH	Cytogenetic Lab	1HE, 3 unstains on coated slides	Minimum 100 nuclei
TB-PCR	Central TB Lab	1 HE, 8 unstains at 8µm for resections, non coated slides	Not applicable
TB-PCR	Central TB Lab	1 HE, 15 unstains at 5µm for biopsies, non coated slides	Not applicable
SSTP	Translational Pathology Centre	Refer to requirement for EGFR	Minimum 30% tumour content

Contact laboratory (Tel: 6321 4008) for further information.

TOP

Search Pathology:

Search Pathology Laboratory Tests

Browse by Category

Search Alphabetically

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z View all

TISSUE PREPARATION FOR POLYMERASE CHAIN REACTION (PCR)/MOLECULAR

Lab Section Category

Description

What's New