INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION PANEL TEST FOR MULTIPLE MYELOMA, B-CELL CHRONIC LYMPHOCYTIC LEUKAEMIA AND MYELODYSPLASTIC SYNDROME

​Many cytogenetic abnormalities are subtle or are found in non-dividing cells that only interphase FISH can be used to elucidate. FISH panels offered by the laboratory include:
1. Multiple Myeloma: Expanded MM FISH panel can be used to detect multiple chromosomal aberrations found in multiple myeloma. See table 2 for probe combination.
2. Combined MM FISH Panel: Inclusive of MM Hyperdiploidy FISH Panel and Expanded MM FISH Panel.
3. B-cell Chronic Lymphocytic Leukaemia: CLL FISH panel can be used to detect multiple aberrations that are  often found in clonal B-cells.
4. Myelodysplastic Syndrome: MDS FISH panel is used to detect MDS.

​Bone marrow in sodium/lithium heparin. For B-cell CLL, the preferred specimen is peripheral blood.

​Fluorescence In Situ Hybridization using direct-labelled FISH DNA probes are hybridized to target loci and analysed under fluorescence microscopy. Refer to Table 2 for the probes used in each panel.

​Positive or Negative for the FISH panel. FISH findings are reported in accordance to the International System for Human Cytogenomic Nomenclature (ISCN, 2020).

​3 ~ 10 days

​Monday – Saturday (office hours)

​The FISH panel test is charged as a separate test from any accompanying cytogenetic test. Four to nine DNA probes, excluding internal control probes, are used in each panel test.