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CINTEC PLUS CYTOLOGY

Synonym(s):

​CINtec PLUS Cytology, Immunocytochemistry

Lab Section Category

Cytology – Gynaecological Cytology (Pap smear)
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Description

CINtec® PLUS Cytology Kit is an immunocytochemistry assay for simultaneous qualitative detection of p16INK4a and Ki-67 proteins in cervical cytology preparations.

Specimen Required

​​1. Cytologic sample collected in PreservCyt® Solution (Hologic Inc.) - see site specific instruction on specimen collection and handling of (A) Gynaecological Cytology for details. (Do not use lubricant as it will affect the quality of the smear prepared).

A cytology gynaecological CPOE request that is properly filled up - see the CPOE request for details. 

OR

2. Archived specimen from Molecular Laboratory that is no more than 6 weeks from collection date may be used.

Alert Cytology lab (6576 7587) of the additional request. Put up a new cytology gynaecological CPOE request that is properly filled up and send the CPOE label to Cytology Lab.

Method

​The CINtec® PLUS Cytology Kit is optimized for use on VENTANA BenchMark GX, XT and ULTRA automated slide strainers. ThinPrep® (Hologic Inc,) slides are prepared on a Thinprep® 2000 processor (Hologic Inc.) according to manufacturers. The p16INK4a and Ki-67 proteins in the cytological specimens are detected using a ready-to-use cocktail of primary monoclonal antibodies which contains a monoclonal mouse antibody directed against human p16INK4a protein (clone E6H4™) and a primary recombinant rabbit antibody against human Ki-67 protein (clone 274-11 AC3).

The staining results are evaluated by light microscopy inspection and are to be interpreted in conjunction with patient’s clinical history and additional diagnostic tests that have been performed.

Turnaround Time

7 working days

Day(s) Test Set up

​Tuesday and Thursday

Change History Notes

  • 01 Nov 2019 4:00 AM

    New test available w.e.f. 05 November 2019

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Last updated on 28 Nov 2024