BK VIRUS QUANTITATIVE PCR

Synonym(s):

BK VIRUS DNA LOAD

Lab Section Category

Molecular Diagnostic - Microbiology

View write-up

Indications

For detection and quantitative measurement of BK virus in urine and blood samples of renal transplant and immunocompromised patients. As an adjunct for the diagnosis of BK virus associated nephropathy or haemorrhagic cystitis.

Specimen Required

(a) Blood: Collect at least 5 mL blood in EDTA containers
(2 full tubes of EDTA blood)

(b) Urine: Collect 2-10 mL of urine (first morning void) in sterile urine bottles

Storage and Transport

Refrigerate sample at 2-8°C if there is a delay in transportation to the laboratory. Do not freeze.

For clients outside SGH, send sample at 2 - 8°C. Plasma may be transported at 2-8°C or frozen at ≤-20°C. Sample must reach laboratory within 24 hours after collection.

Method

Roche COBAS® BKV test
(commercial real-time polymerase chain reaction assay)

Test Results

Detected, Not detected or Inconclusive (quantity reported in copies/mL)

Linear Range
Plasma: 21.5–100,000,000 IU/ml (1.33-8.00 log)
Urine: 200-100,000,000 IU/ml (2.30-8.00 log)

Turnaround Time

1 - 5 days

Day(s) Test Set up

Tuesday AM and Thursday PM (except public holidays)

Change History Notes

05 Feb 2021 8:00 AM

Day(s) Test Set-Up has been last updated on 05/02/21.

09 Sep 2024 8:00 AM

In-house developed BK virus quantitative PCR test upgraded to Roche COBAS® BKV Test with effect from 9 September 2024.

Updates of the following content:
- Specimen Required
- Storage and Transport
- Method
- Test Results
- Day(s) Test Set up

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