Synonym(s):
HSV PCR
For detection and typing of herpes simplex virus (HSV) in patients suspected of having HSV encephalitis, uveitis and HSV infections in other sites.
(a) CSF: At least 0.5 mL of undiluted sample in a sterile and leak-proof screw-capped container without any additives. (b) Swab for vesicles: Use Dacron-tipped swabs. Sample base of lesions vigorously and send dry in swab sheath. (c) Blood: 1-3 mL of EDTA whole blood
Please note that HSV PCR on blood has low yield in most instances of HSV infections (except in disseminated disease or HSV hepatitis). Consider alternatives such as HSV PCR on direct swabs for active lesions, or HSV serology for latent infection.
Refrigerate sample until transfer to laboratory. Do not freeze. Send sample at 2 - 8°C (with an ice-pack). Sample must reach the laboratory within 24 hours after collection.
In-house real-time multiplex Polymerase Chain Reaction (PCR)
Detected, Not Detected or Inconclusive
1 - 4 days; next working day for CSF samples
Mondays and Thursdays (except Public Holidays)
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